episomal factors oct4 Search Results


gene exp gapdh hs99999905 m1  (Thermo Fisher)
99
Thermo Fisher gene exp gapdh hs99999905 m1
Gene Exp Gapdh Hs99999905 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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episomal factors oct4  (Addgene inc)
90
Addgene inc episomal factors oct4
Reprogramming and characterisation of iPSC carrying p.A251T HNF1A variant . A: Overview of the iPSC reprogramming protocol. Somatic fibroblasts were transfected with episomal reprogramming vectors at day 4. After transfection, cells were cultured in N2B27 media with bFGF for 14 days. Media was then switched to stem cell conditions, and colonies were picked around day 21 for further analysis. B: Confocal microscopy images showing <t>OCT4</t> expression in reprogrammed iPSC colonies. Cells were stained with DAPI (blue, 405 nm) to label nuclei and OCT4 (green, 488 nm) to assess pluripotency. Imaging was performed using the Leica Stellaris 8 Confocal Microscope. Co-localisation of DAPI and OCT4 indicates pluripotency. C: Quantification of <t>OCT4-positive</t> cells. OCT4-positive cells were identified by co-localisation with DAPI and quantified using an automated macro. The graph shows the percentage of OCT4-positive cells relative to total cells in the colonies. D: Morphological changes during the differentiation process observed under brightfield microscopy. Day 1: Fibroblast-like cells (top left). Day 12: Intermediate cell morphology (top right). Day 15: iPSC-like colony formation. Day 18: Growth phase, ready for picking. Arrows indicate changes in cell morphology over time. Scale bar = 100 µm. E: Karyotyping analysis of selected iPSC clones. G-banding of chromosomes revealed a normal karyotype with 46 chromosomes, including two X chromosomes, consistent with the donor’s female sex.
Episomal Factors Oct4, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcep4  (Thermo Fisher)
90
Thermo Fisher pcep4
Reprogramming and characterisation of iPSC carrying p.A251T HNF1A variant . A: Overview of the iPSC reprogramming protocol. Somatic fibroblasts were transfected with episomal reprogramming vectors at day 4. After transfection, cells were cultured in N2B27 media with bFGF for 14 days. Media was then switched to stem cell conditions, and colonies were picked around day 21 for further analysis. B: Confocal microscopy images showing <t>OCT4</t> expression in reprogrammed iPSC colonies. Cells were stained with DAPI (blue, 405 nm) to label nuclei and OCT4 (green, 488 nm) to assess pluripotency. Imaging was performed using the Leica Stellaris 8 Confocal Microscope. Co-localisation of DAPI and OCT4 indicates pluripotency. C: Quantification of <t>OCT4-positive</t> cells. OCT4-positive cells were identified by co-localisation with DAPI and quantified using an automated macro. The graph shows the percentage of OCT4-positive cells relative to total cells in the colonies. D: Morphological changes during the differentiation process observed under brightfield microscopy. Day 1: Fibroblast-like cells (top left). Day 12: Intermediate cell morphology (top right). Day 15: iPSC-like colony formation. Day 18: Growth phase, ready for picking. Arrows indicate changes in cell morphology over time. Scale bar = 100 µm. E: Karyotyping analysis of selected iPSC clones. G-banding of chromosomes revealed a normal karyotype with 46 chromosomes, including two X chromosomes, consistent with the donor’s female sex.
Pcep4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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retroviral plasmids  (Addgene inc)
90
Addgene inc retroviral plasmids
Reprogramming and characterisation of iPSC carrying p.A251T HNF1A variant . A: Overview of the iPSC reprogramming protocol. Somatic fibroblasts were transfected with episomal reprogramming vectors at day 4. After transfection, cells were cultured in N2B27 media with bFGF for 14 days. Media was then switched to stem cell conditions, and colonies were picked around day 21 for further analysis. B: Confocal microscopy images showing <t>OCT4</t> expression in reprogrammed iPSC colonies. Cells were stained with DAPI (blue, 405 nm) to label nuclei and OCT4 (green, 488 nm) to assess pluripotency. Imaging was performed using the Leica Stellaris 8 Confocal Microscope. Co-localisation of DAPI and OCT4 indicates pluripotency. C: Quantification of <t>OCT4-positive</t> cells. OCT4-positive cells were identified by co-localisation with DAPI and quantified using an automated macro. The graph shows the percentage of OCT4-positive cells relative to total cells in the colonies. D: Morphological changes during the differentiation process observed under brightfield microscopy. Day 1: Fibroblast-like cells (top left). Day 12: Intermediate cell morphology (top right). Day 15: iPSC-like colony formation. Day 18: Growth phase, ready for picking. Arrows indicate changes in cell morphology over time. Scale bar = 100 µm. E: Karyotyping analysis of selected iPSC clones. G-banding of chromosomes revealed a normal karyotype with 46 chromosomes, including two X chromosomes, consistent with the donor’s female sex.
Retroviral Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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retroviral plasmids pmxs-oct4  (Addgene inc)
90
Addgene inc retroviral plasmids pmxs-oct4
Reprogramming and characterisation of iPSC carrying p.A251T HNF1A variant . A: Overview of the iPSC reprogramming protocol. Somatic fibroblasts were transfected with episomal reprogramming vectors at day 4. After transfection, cells were cultured in N2B27 media with bFGF for 14 days. Media was then switched to stem cell conditions, and colonies were picked around day 21 for further analysis. B: Confocal microscopy images showing <t>OCT4</t> expression in reprogrammed iPSC colonies. Cells were stained with DAPI (blue, 405 nm) to label nuclei and OCT4 (green, 488 nm) to assess pluripotency. Imaging was performed using the Leica Stellaris 8 Confocal Microscope. Co-localisation of DAPI and OCT4 indicates pluripotency. C: Quantification of <t>OCT4-positive</t> cells. OCT4-positive cells were identified by co-localisation with DAPI and quantified using an automated macro. The graph shows the percentage of OCT4-positive cells relative to total cells in the colonies. D: Morphological changes during the differentiation process observed under brightfield microscopy. Day 1: Fibroblast-like cells (top left). Day 12: Intermediate cell morphology (top right). Day 15: iPSC-like colony formation. Day 18: Growth phase, ready for picking. Arrows indicate changes in cell morphology over time. Scale bar = 100 µm. E: Karyotyping analysis of selected iPSC clones. G-banding of chromosomes revealed a normal karyotype with 46 chromosomes, including two X chromosomes, consistent with the donor’s female sex.
Retroviral Plasmids Pmxs Oct4, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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retroviral plasmids producing human oct4, sox2, klf4 c-myc transcription factors  (Addgene inc)
90
Addgene inc retroviral plasmids producing human oct4, sox2, klf4 c-myc transcription factors
Reprogramming and characterisation of iPSC carrying p.A251T HNF1A variant . A: Overview of the iPSC reprogramming protocol. Somatic fibroblasts were transfected with episomal reprogramming vectors at day 4. After transfection, cells were cultured in N2B27 media with bFGF for 14 days. Media was then switched to stem cell conditions, and colonies were picked around day 21 for further analysis. B: Confocal microscopy images showing <t>OCT4</t> expression in reprogrammed iPSC colonies. Cells were stained with DAPI (blue, 405 nm) to label nuclei and OCT4 (green, 488 nm) to assess pluripotency. Imaging was performed using the Leica Stellaris 8 Confocal Microscope. Co-localisation of DAPI and OCT4 indicates pluripotency. C: Quantification of <t>OCT4-positive</t> cells. OCT4-positive cells were identified by co-localisation with DAPI and quantified using an automated macro. The graph shows the percentage of OCT4-positive cells relative to total cells in the colonies. D: Morphological changes during the differentiation process observed under brightfield microscopy. Day 1: Fibroblast-like cells (top left). Day 12: Intermediate cell morphology (top right). Day 15: iPSC-like colony formation. Day 18: Growth phase, ready for picking. Arrows indicate changes in cell morphology over time. Scale bar = 100 µm. E: Karyotyping analysis of selected iPSC clones. G-banding of chromosomes revealed a normal karyotype with 46 chromosomes, including two X chromosomes, consistent with the donor’s female sex.
Retroviral Plasmids Producing Human Oct4, Sox2, Klf4 C Myc Transcription Factors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pmig oct4  (Addgene inc)
93
Addgene inc pmig oct4
Reprogramming and characterisation of iPSC carrying p.A251T HNF1A variant . A: Overview of the iPSC reprogramming protocol. Somatic fibroblasts were transfected with episomal reprogramming vectors at day 4. After transfection, cells were cultured in N2B27 media with bFGF for 14 days. Media was then switched to stem cell conditions, and colonies were picked around day 21 for further analysis. B: Confocal microscopy images showing <t>OCT4</t> expression in reprogrammed iPSC colonies. Cells were stained with DAPI (blue, 405 nm) to label nuclei and OCT4 (green, 488 nm) to assess pluripotency. Imaging was performed using the Leica Stellaris 8 Confocal Microscope. Co-localisation of DAPI and OCT4 indicates pluripotency. C: Quantification of <t>OCT4-positive</t> cells. OCT4-positive cells were identified by co-localisation with DAPI and quantified using an automated macro. The graph shows the percentage of OCT4-positive cells relative to total cells in the colonies. D: Morphological changes during the differentiation process observed under brightfield microscopy. Day 1: Fibroblast-like cells (top left). Day 12: Intermediate cell morphology (top right). Day 15: iPSC-like colony formation. Day 18: Growth phase, ready for picking. Arrows indicate changes in cell morphology over time. Scale bar = 100 µm. E: Karyotyping analysis of selected iPSC clones. G-banding of chromosomes revealed a normal karyotype with 46 chromosomes, including two X chromosomes, consistent with the donor’s female sex.
Pmig Oct4, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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epi5 episomal ipsc reprogramming kit  (Thermo Fisher)
90
Thermo Fisher epi5 episomal ipsc reprogramming kit
Reprogramming and characterisation of iPSC carrying p.A251T HNF1A variant . A: Overview of the iPSC reprogramming protocol. Somatic fibroblasts were transfected with episomal reprogramming vectors at day 4. After transfection, cells were cultured in N2B27 media with bFGF for 14 days. Media was then switched to stem cell conditions, and colonies were picked around day 21 for further analysis. B: Confocal microscopy images showing <t>OCT4</t> expression in reprogrammed iPSC colonies. Cells were stained with DAPI (blue, 405 nm) to label nuclei and OCT4 (green, 488 nm) to assess pluripotency. Imaging was performed using the Leica Stellaris 8 Confocal Microscope. Co-localisation of DAPI and OCT4 indicates pluripotency. C: Quantification of <t>OCT4-positive</t> cells. OCT4-positive cells were identified by co-localisation with DAPI and quantified using an automated macro. The graph shows the percentage of OCT4-positive cells relative to total cells in the colonies. D: Morphological changes during the differentiation process observed under brightfield microscopy. Day 1: Fibroblast-like cells (top left). Day 12: Intermediate cell morphology (top right). Day 15: iPSC-like colony formation. Day 18: Growth phase, ready for picking. Arrows indicate changes in cell morphology over time. Scale bar = 100 µm. E: Karyotyping analysis of selected iPSC clones. G-banding of chromosomes revealed a normal karyotype with 46 chromosomes, including two X chromosomes, consistent with the donor’s female sex.
Epi5 Episomal Ipsc Reprogramming Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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episomal vectors pep4 e02s ck2m en2l  (Addgene inc)
90
Addgene inc episomal vectors pep4 e02s ck2m en2l
Reprogramming and characterisation of iPSC carrying p.A251T HNF1A variant . A: Overview of the iPSC reprogramming protocol. Somatic fibroblasts were transfected with episomal reprogramming vectors at day 4. After transfection, cells were cultured in N2B27 media with bFGF for 14 days. Media was then switched to stem cell conditions, and colonies were picked around day 21 for further analysis. B: Confocal microscopy images showing <t>OCT4</t> expression in reprogrammed iPSC colonies. Cells were stained with DAPI (blue, 405 nm) to label nuclei and OCT4 (green, 488 nm) to assess pluripotency. Imaging was performed using the Leica Stellaris 8 Confocal Microscope. Co-localisation of DAPI and OCT4 indicates pluripotency. C: Quantification of <t>OCT4-positive</t> cells. OCT4-positive cells were identified by co-localisation with DAPI and quantified using an automated macro. The graph shows the percentage of OCT4-positive cells relative to total cells in the colonies. D: Morphological changes during the differentiation process observed under brightfield microscopy. Day 1: Fibroblast-like cells (top left). Day 12: Intermediate cell morphology (top right). Day 15: iPSC-like colony formation. Day 18: Growth phase, ready for picking. Arrows indicate changes in cell morphology over time. Scale bar = 100 µm. E: Karyotyping analysis of selected iPSC clones. G-banding of chromosomes revealed a normal karyotype with 46 chromosomes, including two X chromosomes, consistent with the donor’s female sex.
Episomal Vectors Pep4 E02s Ck2m En2l, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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addgene plasmid  (Addgene inc)
93
Addgene inc addgene plasmid
Reprogramming and characterisation of iPSC carrying p.A251T HNF1A variant . A: Overview of the iPSC reprogramming protocol. Somatic fibroblasts were transfected with episomal reprogramming vectors at day 4. After transfection, cells were cultured in N2B27 media with bFGF for 14 days. Media was then switched to stem cell conditions, and colonies were picked around day 21 for further analysis. B: Confocal microscopy images showing <t>OCT4</t> expression in reprogrammed iPSC colonies. Cells were stained with DAPI (blue, 405 nm) to label nuclei and OCT4 (green, 488 nm) to assess pluripotency. Imaging was performed using the Leica Stellaris 8 Confocal Microscope. Co-localisation of DAPI and OCT4 indicates pluripotency. C: Quantification of <t>OCT4-positive</t> cells. OCT4-positive cells were identified by co-localisation with DAPI and quantified using an automated macro. The graph shows the percentage of OCT4-positive cells relative to total cells in the colonies. D: Morphological changes during the differentiation process observed under brightfield microscopy. Day 1: Fibroblast-like cells (top left). Day 12: Intermediate cell morphology (top right). Day 15: iPSC-like colony formation. Day 18: Growth phase, ready for picking. Arrows indicate changes in cell morphology over time. Scale bar = 100 µm. E: Karyotyping analysis of selected iPSC clones. G-banding of chromosomes revealed a normal karyotype with 46 chromosomes, including two X chromosomes, consistent with the donor’s female sex.
Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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episomal vectors encoding the reprogramming factors oct4, sox2, klf4, lin28a, lmyc, and an shrna targeting p53  (Addgene inc)
90
Addgene inc episomal vectors encoding the reprogramming factors oct4, sox2, klf4, lin28a, lmyc, and an shrna targeting p53
Reprogramming and characterisation of iPSC carrying p.A251T HNF1A variant . A: Overview of the iPSC reprogramming protocol. Somatic fibroblasts were transfected with episomal reprogramming vectors at day 4. After transfection, cells were cultured in N2B27 media with bFGF for 14 days. Media was then switched to stem cell conditions, and colonies were picked around day 21 for further analysis. B: Confocal microscopy images showing <t>OCT4</t> expression in reprogrammed iPSC colonies. Cells were stained with DAPI (blue, 405 nm) to label nuclei and OCT4 (green, 488 nm) to assess pluripotency. Imaging was performed using the Leica Stellaris 8 Confocal Microscope. Co-localisation of DAPI and OCT4 indicates pluripotency. C: Quantification of <t>OCT4-positive</t> cells. OCT4-positive cells were identified by co-localisation with DAPI and quantified using an automated macro. The graph shows the percentage of OCT4-positive cells relative to total cells in the colonies. D: Morphological changes during the differentiation process observed under brightfield microscopy. Day 1: Fibroblast-like cells (top left). Day 12: Intermediate cell morphology (top right). Day 15: iPSC-like colony formation. Day 18: Growth phase, ready for picking. Arrows indicate changes in cell morphology over time. Scale bar = 100 µm. E: Karyotyping analysis of selected iPSC clones. G-banding of chromosomes revealed a normal karyotype with 46 chromosomes, including two X chromosomes, consistent with the donor’s female sex.
Episomal Vectors Encoding The Reprogramming Factors Oct4, Sox2, Klf4, Lin28a, Lmyc, And An Shrna Targeting P53, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/episomal vectors encoding the reprogramming factors oct4, sox2, klf4, lin28a, lmyc, and an shrna targeting p53/product/Addgene inc
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episomal vectors encoding the reprogramming factors oct4, sox2, klf4, lin28a, lmyc, and an shrna targeting p53 - by Bioz Stars, 2026-02
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neon transfection system  (Thermo Fisher)
90
Thermo Fisher neon transfection system
Reprogramming and characterisation of iPSC carrying p.A251T HNF1A variant . A: Overview of the iPSC reprogramming protocol. Somatic fibroblasts were transfected with episomal reprogramming vectors at day 4. After transfection, cells were cultured in N2B27 media with bFGF for 14 days. Media was then switched to stem cell conditions, and colonies were picked around day 21 for further analysis. B: Confocal microscopy images showing <t>OCT4</t> expression in reprogrammed iPSC colonies. Cells were stained with DAPI (blue, 405 nm) to label nuclei and OCT4 (green, 488 nm) to assess pluripotency. Imaging was performed using the Leica Stellaris 8 Confocal Microscope. Co-localisation of DAPI and OCT4 indicates pluripotency. C: Quantification of <t>OCT4-positive</t> cells. OCT4-positive cells were identified by co-localisation with DAPI and quantified using an automated macro. The graph shows the percentage of OCT4-positive cells relative to total cells in the colonies. D: Morphological changes during the differentiation process observed under brightfield microscopy. Day 1: Fibroblast-like cells (top left). Day 12: Intermediate cell morphology (top right). Day 15: iPSC-like colony formation. Day 18: Growth phase, ready for picking. Arrows indicate changes in cell morphology over time. Scale bar = 100 µm. E: Karyotyping analysis of selected iPSC clones. G-banding of chromosomes revealed a normal karyotype with 46 chromosomes, including two X chromosomes, consistent with the donor’s female sex.
Neon Transfection System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Reprogramming and characterisation of iPSC carrying p.A251T HNF1A variant . A: Overview of the iPSC reprogramming protocol. Somatic fibroblasts were transfected with episomal reprogramming vectors at day 4. After transfection, cells were cultured in N2B27 media with bFGF for 14 days. Media was then switched to stem cell conditions, and colonies were picked around day 21 for further analysis. B: Confocal microscopy images showing OCT4 expression in reprogrammed iPSC colonies. Cells were stained with DAPI (blue, 405 nm) to label nuclei and OCT4 (green, 488 nm) to assess pluripotency. Imaging was performed using the Leica Stellaris 8 Confocal Microscope. Co-localisation of DAPI and OCT4 indicates pluripotency. C: Quantification of OCT4-positive cells. OCT4-positive cells were identified by co-localisation with DAPI and quantified using an automated macro. The graph shows the percentage of OCT4-positive cells relative to total cells in the colonies. D: Morphological changes during the differentiation process observed under brightfield microscopy. Day 1: Fibroblast-like cells (top left). Day 12: Intermediate cell morphology (top right). Day 15: iPSC-like colony formation. Day 18: Growth phase, ready for picking. Arrows indicate changes in cell morphology over time. Scale bar = 100 µm. E: Karyotyping analysis of selected iPSC clones. G-banding of chromosomes revealed a normal karyotype with 46 chromosomes, including two X chromosomes, consistent with the donor’s female sex.

Journal: medRxiv

Article Title: Investigating the pathogenicity of the recessive HNF1A p.A251T variant in monogenic diabetes using iPSC-derived beta-like cells

doi: 10.1101/2024.12.10.24318788

Figure Lengend Snippet: Reprogramming and characterisation of iPSC carrying p.A251T HNF1A variant . A: Overview of the iPSC reprogramming protocol. Somatic fibroblasts were transfected with episomal reprogramming vectors at day 4. After transfection, cells were cultured in N2B27 media with bFGF for 14 days. Media was then switched to stem cell conditions, and colonies were picked around day 21 for further analysis. B: Confocal microscopy images showing OCT4 expression in reprogrammed iPSC colonies. Cells were stained with DAPI (blue, 405 nm) to label nuclei and OCT4 (green, 488 nm) to assess pluripotency. Imaging was performed using the Leica Stellaris 8 Confocal Microscope. Co-localisation of DAPI and OCT4 indicates pluripotency. C: Quantification of OCT4-positive cells. OCT4-positive cells were identified by co-localisation with DAPI and quantified using an automated macro. The graph shows the percentage of OCT4-positive cells relative to total cells in the colonies. D: Morphological changes during the differentiation process observed under brightfield microscopy. Day 1: Fibroblast-like cells (top left). Day 12: Intermediate cell morphology (top right). Day 15: iPSC-like colony formation. Day 18: Growth phase, ready for picking. Arrows indicate changes in cell morphology over time. Scale bar = 100 µm. E: Karyotyping analysis of selected iPSC clones. G-banding of chromosomes revealed a normal karyotype with 46 chromosomes, including two X chromosomes, consistent with the donor’s female sex.

Article Snippet: A minimum of 3×10 6 fibroblast cells were electroporated (1650V, 10ms, 3 pulses) with 3ug of three 3 reprogramming plasmids containing the episomal factors Oct4, Sox2, Klf4, Lin28, and L-Myc (OSKM): pCXLE-hSK (Addgene), pCXLE-hUL (Addgene) and pCXLE-hOCT3/4-shp53-F (Addgene) ( ).

Techniques: Variant Assay, Transfection, Cell Culture, Confocal Microscopy, Expressing, Staining, Imaging, Microscopy, Clone Assay